[Electron microscopic study of protoplasts of mesophyll tobacco. I. Ultrastructural and cytochemical study of tobacco protoplasts at early stages of cultivation]
[Article in
Russian]
- PMID: 910296
Item in Clipboard
[Electron microscopic study of protoplasts of mesophyll tobacco. I. Ultrastructural and cytochemical study of tobacco protoplasts at early stages of cultivation]
[Article in
Russian]
Tsitol Genet.
1977 May-Jun.
Abstract
Ultrastructural changes in tobacco mesophyll protoplasts during the first three days of cultvation were studied. Localization of catalase and peroxidase activities in the freshly isolated protoplasts and 2 days after their cultivation was detected by the cytochemical 3,3'-diaminobenzidine method. A conclusion is drawn that the observed changes in the fine structure and localization of the enzymic activity are due to the following processes: reparation of disturbances caused by the isolation procedure, regeneration of the cell wall and rapid dedifferentiation of the initial parenchymatic leaf cells.
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