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. 1977 May-Jun;11(3):259-67.

[Electron microscopic study of protoplasts of mesophyll tobacco. I. Ultrastructural and cytochemical study of tobacco protoplasts at early stages of cultivation]

[Article in Russian]
N V Belitser, V A Sidorov, V A Tarasenko, Iu Iu Gleva, K M Sytnik
  • PMID: 910296

[Electron microscopic study of protoplasts of mesophyll tobacco. I. Ultrastructural and cytochemical study of tobacco protoplasts at early stages of cultivation]

[Article in Russian]
N V Belitser et al. Tsitol Genet. 1977 May-Jun.

Abstract

Ultrastructural changes in tobacco mesophyll protoplasts during the first three days of cultvation were studied. Localization of catalase and peroxidase activities in the freshly isolated protoplasts and 2 days after their cultivation was detected by the cytochemical 3,3'-diaminobenzidine method. A conclusion is drawn that the observed changes in the fine structure and localization of the enzymic activity are due to the following processes: reparation of disturbances caused by the isolation procedure, regeneration of the cell wall and rapid dedifferentiation of the initial parenchymatic leaf cells.

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