Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997 Feb;16(2):162-4.
doi: 10.1007/BF01709478.

Evaluation of rapid molecular methods for detection of clarithromycin resistance in Helicobacter pylori

F Szczebara  1 , L Dhaenens, P Vincent, M O Husson
Affiliations

Evaluation of rapid molecular methods for detection of clarithromycin resistance in Helicobacter pylori

F Szczebara et al. Eur J Clin Microbiol Infect Dis. 1997 Feb.

Abstract

Resistance of Helicobacter pylori to clarithromycin is due to point mutations at position A2143 or A2144 of the rrnH 23S rRNA gene, each mutation creating an additional restriction site for BsaI or MboII. A procedure combining PCR and RFLP analysis was evaluated for detection of these mutations using primers specific for the 23S rRNA gene, and BsaI and MboII enzymes. All clarithromycin-resistant isolates (8/8), as defined by the MIC, were found to be resistant by PCR-RFLP. No clarithromycin-sensitive isolates (14/14) gave a positive reaction.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Antimicrob Agents Chemother. 1996 Feb;40(2):477-80 - PubMed
    1. Eur J Clin Microbiol Infect Dis. 1996 Jun;15(6):484-8 - PubMed
    1. Eur J Clin Microbiol Infect Dis. 1992 Sep;11(9):777-81 - PubMed
    1. Antimicrob Agents Chemother. 1995 Mar;39(3):577-85 - PubMed
    1. J Antimicrob Chemother. 1996 Apr;37(4):639-43 - PubMed

MeSH terms

LinkOut - more resources