Influence of dietary arachidonic acid on metabolism in vivo of 8cis,11cis,14-eicosatrienoic acid in humans

Abstract

This study investigated the influence of dietary arachidonic acid (20:4n-6) on delta 5 desaturation and incorporation of deuterium-labeled 8cis,11 cis, 14-eicosatrienoic acid (20:3n-6) into human plasma lipids. Adult male subjects (n = 4) were fed diets containing either 1.7 g/d (HI20:4 diet) or 0.21 g/d (LO20:4 diet) of arachidonic acid for 50 d and then dosed with a mixture containing ethyl esters of 20:3n-6[d4] and 18:1n-9[d2]. A series of blood samples was sequentially drawn over a 72-h period, and methyl esters of plasma total lipid, triacylglycerol, phospholipids, and cholesteryl ester were analyzed by gas chromatography-mass spectrometry. Based on the concentration of 20:3n6[d4] in total plasma lipid, the estimated conversion of 20:3n-6[d4] to 20:4n-6[d4] was 17.7 +/- 0.79% (HI20:4 diet) and 2.13 +/- 1.44% (LO20:4 diet). The concentrations of 20:4n-6[d4] in total plasma lipids from subjects fed the HI20:4 and LO20:4 diets were 2.10 +/- 0.6 and 0.29 +/- 0.2 mumole/mL plasma/mmole of 20:3n-6[d4] fed/kg of body weight. These data indicate that conversion of 20:3n-6[d4] to 20:4n-6[d4] was stimulated 7-8-fold by the HI20:4 diet. Phospholipid acyltransferase was 2.5-fold more selective for 20:3n-6[d4] than 18:1n-9[d2], and lecithin:cholesterol acyltransferase was 2-fold more selective for 18:1n-9[ds] than 20:3n-6[d4]. These differences in selectivity were not significantly influenced by diet. Absorption of ethyl 20:3n-6[d4] was about 33% less than ethyl 18:1n-9[d2]. The sum of the n-6 retroconversion products from 20:3n-6[d4] in total plasma lipids was about 2% of the total deuterated fatty acids. Neither absorption nor retroconversion appears to be influenced by diet.