In postmeiotic male germ cells poly (A) shortening accompanies translation of mRNA encoding gamma enteric actin but not cytoplasmic beta and gamma actin mRNAs

Abstract

In the mammalian testis the cytoplasmic beta and gamma actins are expressed in all stages of germ-cell differentiation, whereas gamma enteric actin is expressed in germ cells solely in postmeiotic stages. Northern blot analysis of mouse testicular RNAs reveals actin mRNAs of about 2.1, 1.5, and 1.4 kB. The 2.1-kB mRNAs encode the cytoplasmic beta and gamma actins, whereas the two faster-migrating actin mRNAs encode gamma enteric actin. When postmitochondrial mouse testis extracts are fractionated by sucrose gradient centrifugation, the 1.5-kB gamma enteric actin mRNA is primarily found in the nonpolysomal fraction, whereas the 1.4-kB gamma enteric actin is polysomal. When the poly (A) tails are removed, the nonpolysomal and polysomal gamma enteric actin mRNAs both migrate at 1.3 kB, indicating that the difference in electrophoretic mobilities of the two gamma enteric actin mRNAs is caused by poly (A) length differences. The nonpolysomal and polysomal forms of the cytoplasmic beta and gamma actins show similar electrophoretic mobilities before and after deadenylation. Sequence comparison of the 3' untranslated region of the mouse gamma enteric actin to the 3' untranslated regions of other testicular mRNAs that undergo partial deadenylation reveals three highly-conserved sequence elements. These data demonstrate that the poly (A) shortening of polysomal mRNAs previously seen only with testis-specific mRNAs that are stored as mRNPs also occurs with mRNAs of widely-expressed genes that are expressed in postmeiotic male germ cells. The mRNAs all contain specific conserved sequence elements in their 3' untranslated regions.