CPC-PTA section staining of acid glycans

Abstract

A cetylpyridinium chloride-phosphotungstic acid procedure suggested by Kelényi and Kiss (1976) for the staining of mast cell granules in ultrathin sections was slightly modified with regard to pretreatment, pH of incubation, and rinsing of sections. The method also proved suitable for the demonstration of various mucosubstances and of cartilage proteoglycan aggrebates.