Long-term monitoring of extracellular dopamine concentration in the rat striatum by a repeated microdialysis procedure

Abstract

This study examined a protocol for repeated measurement of the extracellular dopamine (DA) concentration in the rat striatum by microdialysis. Rats were implanted with a guide cannula in the striatum and the probe was inserted on each dialysing day, i.e. ten times over a 23 day period. During this period the animals were submitted to a control saline treatment. DA concentration was measured using the no-net-flux method. In these conditions, DA concentration remained remarkably constant over the 23 day period. The histological analysis using glial fibrillary acidic protein (GFAP), dopamine (DA) and tyrosine hydroxylase (TH) immunocytochemistry showed a moderate gliosis and a discrete increase of immunoreactivity of catecholaminergic fibres around the probe implantation site. This increase is probably related to a plasticity of the dopaminergic system in response to the lesion due to the probe implantations. This study shows that such a paradigm makes possible to measure the whole time course of the DA concentration in the rat striatum during chronic treatments with psychoactive drugs such cocaine or other compounds acting in the nigrostriatal system.