doi: 10.1002/(SICI)1097-0061(19970330)13:4<391::AID-YEA92>3.0.CO;2-Q.
Identification of gene encoding a putative RNA-helicase, homologous to SKI2, in chromosome VII of Saccharomyces cerevisiae
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- PMID: 9133744
- DOI: 10.1002/(SICI)1097-0061(19970330)13:4<391::AID-YEA92>3.0.CO;2-Q
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Identification of gene encoding a putative RNA-helicase, homologous to SKI2, in chromosome VII of Saccharomyces cerevisiae
Yeast.
.
Abstract
We have determined the nucleotide sequence of a segment of VII of the yeast Saccharomyces cerevisiae contained in the cosmid clone pEGH101 for a total of 7 kbp. This sequence contains a large open reading frame (ORF) called G9365, coding for a protein of 1967 amino acids that shows a significant homology with the product of the SKI2 gene of S. cerevisiae and contains domains characteristics of RNA-helicases. The ORF is transcribed in vegetative cells but it is not essential for viability as demonstrated by gene disruption.
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