Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Classical Article
. 1997 Feb;120(4 Suppl):3-26; discussion 1-2.
doi: 10.1111/j.1476-5381.1997.tb06771.x.

Observations on the isolated phrenic nerve diaphragm preparation of the rat. 1946

E Bülbring
Classical Article

Observations on the isolated phrenic nerve diaphragm preparation of the rat. 1946

E Bülbring. Br J Pharmacol. 1997 Feb.
No abstract available

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Perspex electrodes.
Fig. 2
Fig. 2
Contractions of rat's diaphragm. (a) and (b) stimulation of phrenic nerve with open electrodes, (c) with fluid electrodes. The effect of 50 μg. adrenaline on the muscle response to maximal (a) and submaximal single shocks (b) and (c).
Fig. 3
Fig. 3
Rat's phrenic nerve-diaphragm preparation. Comparison of the adrenaline effect at different rates of stimulation: (a) maximal, 6 per min.; (b) submaximal, 6 per min.; (c) submaximal 18 per min.; (d) muscle fatigued by maximal stimulation 18 per min.
Fig. 4
Fig. 4
Rat's phrenic nerve-diaphragm preparation. Maximal single shocks 9 per min. Prostigmine causes an increase of muscle contractions, and after a wash out (W) adrenaline causes a further augmentation.
Fig. 5
Fig. 5
Rat's phrenic nerve-diaphragm preparation. Maximal single shocks 18 per min. 0.1 μg. prostigmine had been given beforehand and was washed out; in (a) 10 μg. adrenaline (A) caused a rise in muscle contractions. In (b) when 0.1 μg. prostigmine (P) was not washed out 10 μg. adrenaline (A) caused a fall in muscle contractions.
Fig. 6
Fig. 6
Rat's phrenic nerve-diaphragm preparation. Effect of adrenaline (a) after and (b) before washing out a previous dose of prostigmine. Maximal single shocks 7 per min. P = 0.1 μg. prostigmine; W = wash, A = 10 μg. adrenaline
Fig. 7
Fig. 7
Rat's phrenic nerve-diaphragm preparation. Maximal single shocks 14 per min. P = 0.1 μg. prostigmine, W = wash, A = 20 μg. adrenaline. For description see text.
Fig. 8
Fig. 8
Rat's phrenic nerve-diaphragm preparation. Maximal single shocks 5 per min. W. = wash. (a) shows the depression caused by 0.1 μg. eserine on a fresh preparation, (b) the effect of 50 μg. adrenaline 35 min. later, (c) shows the augmentation caused by 0.2 μg. eserine in the same preparation 3 hours later, and the further increase due to 50 μg. adrenaline after eserine had bean washed out. In (d) 50μg. adrenaline was added without removing the eserine; note the similarity between (d) and (b).
Fig. 9
Fig. 9
Rat's phrenic nerve-diaphragm preparation. Maximal single shocks 6 per min. In a fresh preparation (a) adrenaline caused a depression in the presence of eserine; 3 hours later (b) it caused augmentation.
Fig. 10
Fig. 10
Rat's phrenic nerve-diaphragm preparation. Maximal single shocks 8 per min. The effect of atropine on (a) the contractions of a fresh muscle, (b) on the contractions increased by 0.2 μg. eserine.
Fig. 11
Fig. 11
Similar to Fig. 10. Maximal single shocks 9 per min. The effect of atropine on (a) the contractions of a fresh muscle, (b) on the contractions depressed by 20 μg. eserine.
Fig. 12
Fig. 12
Rat's phrenic nerve-diaphragm preparation. Maximal single shocks at rates per min. indicated by figures. Note that after eserine (a) contractions decline during fast and recover during slow rates, while after curarine (b) contractions decline during slow and increase during rapid rates.
Fig. 13
Fig. 13
Maximal single shocks at 50, 5 and 18 per min. in a fully eserinized and atropinized phrenic nerve-diaphragm preparation.
Fig. 14
Fig. 14
Rat's phrenic nerve-diaphragm preparation. Maximal single shocks 8 per min. The effect of 1 mg. procaine on the size of muscle contractions (a) when increased. (b) when depressed by prostigmine.
Fig. 15
Fig. 15
Rat's phrenic nerve-diaphragm preparation. Maximal single shocks 5 per min: 2 μg. prostigmine present throughout. The effect of increasing the rate of stimulation to 28 per min. and of 0.5 mg. acetylcholine (a) before and (b) after 35 μg. curarine.
Fig. 16
Fig. 16
Similar to Fig. 15. Maximal single shocks 6 per min.; 2 μg. prostigmine present throughout. The effect of increasing the rate of stimulation to 18 per min., and of 0.5 mg. acetylcholine (a) before and (b) after 2 mg. atropine; (c) after 35 mg. curarine: (d) after 2 mg. atropine.
Fig. 17
Fig. 17
Similar to Fig. 15. Maximal single shocks 6 per min.; 2 μg. prostigmine present throughout. The effect of increasing the rate of stimulation to 28 per min., and of 0.5 mg. acetylcholine (a) before and (b) after 2 mg. procaine; (c) after washing out; (d) after 2 mg. atropine, and (e) after washing out; (f) after 35μg. curarine.
See this image and copyright information in PMC

References

    1. Abdon NA. Acta Physiol. Scand. 1940;1:153.
    1. Bacq ZM, Brown GL. J. Physiol. 1937;89:45. - PMC - PubMed
    1. Brown GL. J. Physiol. 1937;89:438. - PMC - PubMed
    1. Brown GL, Feldberg W. J. Physiol. 1936;88:265. - PMC - PubMed
    1. Briscoe G. Lancet. 1936;1:469.

Substances

Personal name as subject

LinkOut - more resources