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. 1997 Apr 17;233(2):295-9.
doi: 10.1006/bbrc.1997.6447.

The presence of the 50-kDa subunit of dynactin complex in the nerve growth cone

T K Abe  1 , H Tanaka, T Iwanaga, S Odani, R Kuwano
Affiliations

The presence of the 50-kDa subunit of dynactin complex in the nerve growth cone

T K Abe et al. Biochem Biophys Res Commun. .

Abstract

Membrane proteins in growth cone-enriched and growth cone-non-enriched fractions prepared from neonatal mouse brain were separated by lectin-affinity and ion exchange chromatographies, 2D-PAGE, and SDS-PAGE. Partial amino acid sequences of the proteins concentrated in the growth cone-enriched fraction were determined. We found that one such protein, gmp23-48k, corresponds to the 50-kDa subunit (p50) of the dynactin complex. An antibody raised against gmp23-48k strongly reacted with growth cones of differentiated neuronal precursor cells. Immunoblot analyses revealed that gmp23-48k was present both in membrane and in soluble fractions of neonatal brain. However, the amount of gmp23-48k in the membrane fraction greatly decreased in adult brain. These results suggest a special role of membrane-associated gmp23-48k/p50 in synapse formation during brain development.

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