doi: 10.1007/BF00493331.
Electron microscopic dry-mounting radioautography for diffusible compounds by means of ultracryotomy
- PMID: 914660
- DOI: 10.1007/BF00493331
Item in Clipboard
Electron microscopic dry-mounting radioautography for diffusible compounds by means of ultracryotomy
Histochemistry.
.
Abstract
Cultured HeLa cells or mouse liver and pancreas tissues were labeled with 3H-thymidine, -uridine or -glycine for varying periods in vitro, frozen in liquid nitrogen and cut on an LKB ultrotome equipped with LKB Cryokit. Dry ultrathin sections were mounted on grid meshes and were either air-dried, freeze-substituted or freeze-dried, and were covered with dry films of radioautographic emulsions, exposed, developed, stained and were observed in electron microscopes. After a number of trials, it was possible to obtain fairly good preservation of both cell structure and radioisotopes by means of freeze-dried and dry-mounted ultrathin frozen sections. However, the results are not completely satisfactory at the present time.
Similar articles
-
Three-dimensional high voltage electron microscopy of thick biological specimens.Micron. 2001 Jun;32(4):387-404. doi: 10.1016/s0968-4328(00)00005-6. Micron. 2001. PMID: 11070359
-
A new technique for electron microscopic dry-mounting radioautography of soluble-compounds.Histochemie. 1969;18(3):241-9. doi: 10.1007/BF00306170. Histochemie. 1969. PMID: 4898217 No abstract available.
-
Morphometry in anatomy: image analysis on fine structure and histochemical reactions with special reference to radioautography.Ital J Anat Embryol. 1995;100 Suppl 1:591-603. Ital J Anat Embryol. 1995. PMID: 11322340
-
Macromolecular synthesis in the livers of aging mice as revealed by electron microscopic radioautography.Prog Histochem Cytochem. 2010 Apr;45(1):1-79. doi: 10.1016/j.proghi.2009.12.001. Epub 2010 Jan 8. Prog Histochem Cytochem. 2010. PMID: 20207258 Review.
-
Preparation and transfer of ultrathin frozen-hydrated and freeze-dried cryosections for microanalysis in scanning transmission electron microscopy.Scan Electron Microsc. 1982;(Pt 3):1205-14. Scan Electron Microsc. 1982. PMID: 6763328 Review.
Cited by
-
Electron Microscopic Radioautographic Study on the Protein Synthesis in the Pancreas of Aging Mice With Special Reference to Mitochondria.Gastroenterology Res. 2011 Jun;4(3):114-121. doi: 10.4021/gr310e. Epub 2011 May 20. Gastroenterology Res. 2011. PMID: 27942325 Free PMC article.
-
Incorporation of 3H-befunolol (beta-blocking agent) into melanin granules of ocular tissues in the pigmented rabbits. I. Light microscopic radioautography.Histochemistry. 1981 Dec;73(3):371-5. doi: 10.1007/BF00495651. Histochemistry. 1981. PMID: 6120151
-
Cell Aging of Mouse Gastrointestinal Tract Observed by Light and Electron Microscopic Radioautography.Gastroenterology Res. 2014 Aug;7(3-4):81-92. doi: 10.14740/gr617e. Epub 2014 Jul 31. Gastroenterology Res. 2014. PMID: 27785275 Free PMC article. Review.