Sequence of the A-protein of coliphage MS2. II. Isolation and sequence determination of chymotryptic peptides
- PMID: 914837
Sequence of the A-protein of coliphage MS2. II. Isolation and sequence determination of chymotryptic peptides
Abstract
A total of 94 different peptides have been isolated from chymotryptic digests of underivatized (35 mg) and of reduced, carboxymethylated and citraconylated (48 mg) A-protein. Their amino acid sequences provide information for 375 residues of the total of 393 amino acids of the A-protein polypeptide chain. Abnormal specificity of chymotrypsin in the digestion of citraconylated A-protein was observed.
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