Studies of the secretory process in the mammalian exocrine pancreas. I. The condensing vacuoles
- PMID: 914894
- PMCID: PMC2111565
- DOI: 10.1083/jcb.75.1.148
Studies of the secretory process in the mammalian exocrine pancreas. I. The condensing vacuoles
Abstract
Phosphatase cytochemistry was used to distinguish between the Golgi apparatus and GERL (considered as a specialized region of endoplasmic reticulum [ER] at the inner [trans] aspect of the Golgi stack) in pancreatic exocrine cells of guinea pig, rat, rabbit, and hamster. The trans element of the Golgi stack exhibits thiamine pyrophosphatase (TPPase) but no acid phosphatase (AcPase) activity. In contrast, GERL shows AcPase but no TPPase activity. The nascent secretory granules, or condensing vacuoles, are expanded cisternal portions of GERL. Continuities of condensing vacuoles with rough ER are suggested, and it is proposed that some secretory components may have direct access to the condensing vacuoles from ER. Connections of Golgi apparatus with GERL were not seen.
Similar articles
-
Fine structural localization of thiamine pyrophosphatase and acid phosphatase activities in the mouse pancreatic acinar cell.Histochemistry. 1979;64(3):287-95. doi: 10.1007/BF00495029. Histochemistry. 1979. PMID: 521317
-
The corpus luteum of the guinea pig. III. Cytochemical studies on the Golgi complex and GERL during normal postpartum regression of luteal cells, emphasizing the origin of lysosomes and autophagic vacuoles.J Cell Biol. 1978 Oct;79(1):59-73. doi: 10.1083/jcb.79.1.59. J Cell Biol. 1978. PMID: 701378 Free PMC article.
-
The corpus luteum of the guinea pig. II. Cytochemical studies on the Golgi complex, GERL, and lysosomes in luteal cells during maximal progesterone secretion.J Cell Biol. 1978 Oct;79(1):45-58. doi: 10.1083/jcb.79.1.45. J Cell Biol. 1978. PMID: 701377 Free PMC article.
-
Enzyme modulation of the Golgi apparatus and GERL: a cytochemical study of parotid acinar cells.J Histochem Cytochem. 1983 Aug;31(8):1041-8. doi: 10.1177/31.8.6134769. J Histochem Cytochem. 1983. PMID: 6134769 Review.
-
The endoplasmic reticulum: a cytochemist's view (a review).Proc Natl Acad Sci U S A. 1976 Aug;73(8):2781-7. doi: 10.1073/pnas.73.8.2781. Proc Natl Acad Sci U S A. 1976. PMID: 183210 Free PMC article. Review.
Cited by
-
Ultrastructural identification of sulphated glycoconjugates in the Golgi apparatus in human colonic absorptive cells.Histochemistry. 1989;92(1):73-9. doi: 10.1007/BF00495020. Histochemistry. 1989. PMID: 2670847
-
A morphometrical study of the exocrine pancreatic cell in fasted and fed frogs.J Cell Biol. 1979 Mar;80(3):692-707. doi: 10.1083/jcb.80.3.692. J Cell Biol. 1979. PMID: 313397 Free PMC article.
-
Different aspects of membrane differentiation at the inner side (GERL) of the Golgi apparatus in rabbit luteal cells.Histochem J. 1979 Jul;11(4):399-416. doi: 10.1007/BF01002768. Histochem J. 1979. PMID: 500396
-
Fine structural localization of thiamine pyrophosphatase and acid phosphatase activities in the mouse pancreatic acinar cell.Histochemistry. 1979;64(3):287-95. doi: 10.1007/BF00495029. Histochemistry. 1979. PMID: 521317
-
Recovery of surface membrane in anterior pituitary cells. Variations in traffic detected with anionic and cationic ferritin.J Cell Biol. 1978 Jun;77(3):R35-42. doi: 10.1083/jcb.77.3.r35. J Cell Biol. 1978. PMID: 567224 Free PMC article.
