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. 1977 Oct;6(4):392-5.
doi: 10.1128/jcm.6.4.392-395.1977.

Effect of freezing on the viability of Mycobacterium paratuberculosis in bovine feces

W D Richards, C O Thoen

Effect of freezing on the viability of Mycobacterium paratuberculosis in bovine feces

W D Richards et al. J Clin Microbiol. 1977 Oct.

Abstract

Three bovine fecal specimens were cultured for Mycobacterium paratuberculosis before freezing and after frozen storage at -70 degrees C for 3 and 15 weeks. The losses in viability from 0 to 3 weeks of storage were significant (P = 0.01) for all three samples. The losses in viability between 3 and 15 weeks of storage were not significant (P greater than 0.05) for two specimens in which the M. paratuberculosis occurred naturally, but the loss was significant (P = 0.01) in a simulated specimen (M. paratuberculosis culture added to feces from a healthy cow).

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References

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    1. Am J Vet Res. 1968 Aug;29(8):1533-8 - PubMed
    1. Can Vet J. 1975 Feb;16(2):33-43 - PubMed

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