Absorption spectra of corneas in the far ultraviolet region

Abstract

Purpose: To study the corneal absorption in the far ultraviolet (UV) region between 260 and 190 nm.

Methods: Thirty-four corneal samples of thickness near 20 microns were obtained from 18 porcine corneas and six human corneas with a microtome-cryostat. The authors conducted absorbance measurements of the sectioned corneal samples supported by two UV optical windows from 350 to 190 nm using a dual-beam spectrophotometer. Three whole porcine corneas were used to study the effect of freezing on the absorbance from 350 to near 290 nm.

Results: The absorption spectra of porcine and human corneas from 350 to 190 nm were measured and three segments in the spectrum between 260 and 190 nm have been identified. The linear absorption coefficients were determined to be 2300 +/- 330 (cm-1) at 210 nm and 2410 +/- 370 (cm-1) at 193 nm for the porcine corneas and 2320 +/- 470 (cm-1) at 210 nm and 2340 +/- 150 (cm-1) at 193 nm for the human corneas.

Conclusions: A "window of ablation" in the far UV region between 220 and 190 nm has been identified in which short laser pulses of similar durations and different wavelengths may be interchangeable to ablate the corneal surface with similar characteristics.