Antigen-specific suppressor factors produced by T cell hybridomas for delayed-type hypersensitivity

Abstract

This study describes the generation of T hybridoma lines which secret factors specifically suppressing delayed-type hypersensitivity (DTH) to sheep red blood cells (SRBC). AKR strain-derived T lymphoma BW 5147 cells were fused with spleen cells from mice primed with SRBC and containing antigen-specific T suppressor cells for DTH. Supernants from the derived hybridomas were tested for suppression of either expression of induction of DTH to SRBC. Six lines produced specific suppressor activity for the expression of DTH; 4 lines produced suppressor activity for the induction of DTH of which only one line was antigen-specific. These lines were passaged in normal AKR mice, and the serum obtained had activity up to 10(-4) dilution. The factor was effective across the H-2 barrier.