Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997 Apr;35(4):923-7.
doi: 10.1128/jcm.35.4.923-927.1997.

Propagation of granulocytic Ehrlichia spp. from human and equine sources in HL-60 cells induced to differentiate into functional granulocytes

R Heimer  1 , A Van Andel, G P Wormser, M L Wilson
Affiliations

Propagation of granulocytic Ehrlichia spp. from human and equine sources in HL-60 cells induced to differentiate into functional granulocytes

R Heimer et al. J Clin Microbiol. 1997 Apr.

Abstract

Ehrlichia spp. from human and equine sources in the northeastern Unites States were detected by PCR, isolated, and propagated in the HL-60 promyelocytic leukemia cell line. Growth of Ehrlichia from both equine and human sources was enhanced by addition of retinoic acid, which causes granulocytic differentiation of the HL-60 cells. DNA sequencing of a portion of the 16S rDNA gene supported the hypothesis that the same pathogen was responsible for both equine and human granulocytic ehrlichiosis.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1980 May;77(5):2936-40 - PubMed
    1. Exp Cell Res. 1987 Jan;168(1):247-54 - PubMed
    1. Clin Microbiol Rev. 1991 Jul;4(3):286-308 - PubMed
    1. J Clin Microbiol. 1991 Dec;29(12):2838-42 - PubMed
    1. Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2699-702 - PubMed

Publication types