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. 1997 Apr;35(4):995-8.
doi: 10.1128/jcm.35.4.995-998.1997.

Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model

L Monteiro  1 , D Bonnemaison, A Vekris, K G Petry, J Bonnet, R Vidal, J Cabrita, F Mégraud
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Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model

L Monteiro et al. J Clin Microbiol. 1997 Apr.

Abstract

A model was developed to study inhibitors present in feces which prevent the use of PCR for the detection of Helicobacter pylori. A DNA fragment amplified with the same primers as H. pylori was used to spike samples before extraction by a modified QIAamp tissue method. Inhibitors, separated on an Ultrogel AcA44 column, were characterized. Inhibitors in feces are complex polysaccharides possibly originating from vegetable material in the diet.

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