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Clinical Trial
. 1997 Jan;12(1):7-15.
doi: 10.3904/kjim.1997.12.1.7.

Differences in immunophenotyping of mucosal lymphocytes between ulcerative colitis and Crohn's disease

H B Lee  1 , J H Kim, C Y Yim, D G Kim, D S Ahn
Affiliations
Clinical Trial

Differences in immunophenotyping of mucosal lymphocytes between ulcerative colitis and Crohn's disease

H B Lee et al. Korean J Intern Med. 1997 Jan.

Abstract

Objectives: Immunologic studies have characterized the numbers and types of inflammatory cells in diseased inflammatory bowel disease (IBD) mucosa but have yielded conflicting results regarding intestinal lymphocytes activation in IBD. We investigated the levels of lymphocytes subsets, interleukin-2 receptor, transferrin receptor, and T cell receptors in mainly isolated lamina propria lymphocytes. Including intraepithelial lymphocytes of normal colonic mucosa or IBD (ulcerative colitis and Crohn's disease) mucosa to understand the pathogenesis of IBD. We have results from this study.

Results: 1) In comparing ulcerative colitis with control, IL-2R (p < 0.05), TR (p < 0.01), and CD3/HLA-DR (< 0.05) showed a significant increase. 2) In comparing Crohn's disease with control, CD3 (P < 0.05), TCR alpha/beta (p < 0.01) and TCR gamma/delta (p < 0.05) showed a significant decrease. 3) In comparing Crohn's disease with ulcerative colitis, CD19 (p < 0.01), TR (p < 0.01), TCR alpha/beta (p < 0.01) and TCR gamma/delta (p < 0.05) showed a significant decrease.

Conclusion: From these results, there are increased T cell markers, IL-2R, TR, and CD3/HLA-DR in UC, but differently, decreased CD3, TCR alpha/beta and TCR gamma/delta in CD compared with control. In addition, definitive differences in lymphocytes markers, CD19, TR, TCR alpha/beta and TCR gamma/delta, which are higher in UC than in CD, may elucidate the different immunopathogenesis between UC and CD.

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Figures

Fig. 1.
Fig. 1.
Percentage of CD19+ cells of the intestinal mucosal lymphocyte from mucosal biopsies. Significantly lesser infiltration in CD than in UC (p<0.01).
Fig. 2.
Fig. 2.
Percentage of CD3+ Cells of the intestinal mucosal lymphocyte from mucosal biopsies. Significantly lesser infiltration in CD than in normal control (p<0.05).
Fig. 3.
Fig. 3.
Percentage of CD25+ cells of the intestinal mucosal lymphocyte from mucosal biopsies. Significantly greater infiltration in CD than in normal control (p<0.05).
Fig. 4.
Fig. 4.
Percentage of CD3/HLA-DR+ cells of the intestinal mucosal lymphocyte from mucosal biopsies. Significantly greater infiltration in UC than in normal control (p<0.05).
Fig. 5.
Fig. 5.
Percentage of TR+ cells of the intestinal mucosal lymphocyte from mucosal biopsies. Significantly greater infiltration in UC than in normal control (p<0.01), and lesser infiltration in CD than in UC (p<0.01).
Fig. 6.
Fig. 6.
Percentage of TCR α/β+ cells of the intestinal mucosal lymphocyte from musosal biopsies. Significantly lesser infiltration in CD than in normal control (p<0.01), and in UC(p<0.01).
Fig. 7.
Fig. 7.
Percentage of TCR γ/δ+ cells of the intestinal musosal lymphocyte from mucosal biopsies. Significantly lesser infiltration in CD than in normal control (p<0.05) and in UC (p<0.05).
See this image and copyright information in PMC

References

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