Detection of polymorphic FGF receptor 1 mRNA in bovine lens epithelium by PCR
- PMID: 9161712
- DOI: 10.1080/15216549700202261
Detection of polymorphic FGF receptor 1 mRNA in bovine lens epithelium by PCR
Abstract
The expression of FGFR 1, 2 and 3 but not FGFR 4 was detected in bovine lens epithelial cells. FGFR 1 expression of both the 2-lg and 3-lg forms was detected by a difference in the lengths of the PCR products. Furthermore, mRNA which included or excluded six nucleotides corresponding to two amino acids in the acid box region of FGFR 1 was detected by a difference in mobility of the homo- and hetero-duplex strands formed in the PCR reaction.
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