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. 1997 Jan-Feb;45(1-2):1-7.

Fluid shear stress increases the release of platelet derived growth factor BB (PDGF BB) by aortic endothelial cells

C Aromatario  1 , A V Sterpetti, R Palumbo, A L Patrizi, A Di Carlo, P Proietti, M B Guglielmi, A Cavallaro, L Santoro-D'Angelo, A Cucina
Affiliations
  • PMID: 9167422

Fluid shear stress increases the release of platelet derived growth factor BB (PDGF BB) by aortic endothelial cells

C Aromatario et al. Minerva Cardioangiol. 1997 Jan-Feb.

Abstract

Objectives: The aim of this study was to determine the correlation between shear stress and the release of Platelet Derived Growth Factor (PDGF BB) by aortic endothelial cells.

Design and setting: Laboratory in vitro study.

Materials: Bovine aortic endothelial cells were seeded in fibronectin-coated cylinders at 1.0 x 10(6) cells/tube and allowed to reach confluence and to adhere for 48 hours. The experimental groups were subjected to nonpulsatile, laminar flow of 50, 100, 150 ml/min in polystyrene cylinders (i.d. 10 mm) of a closed circulatory loop giving a shear stress on the endothelial cells of 3, 6, 9 dyn/cm2. The control group was subjected to similar incubation conditions without flow.

Outcome measures: The release of PDGF BB by endothelial cells was measured by ELISA and Western Blot Analysis.

Results: Shear stress increased significantly (p < 0.01) the release of PDGF BB by endothelial cells.

Conclusions: PDGF BB release by endothelial cells may be one of the mechanisms linking hemodynamic forces and adaptation of blood vessels wall.

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