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. 1997 Jun;63(6):2442-5.
doi: 10.1128/aem.63.6.2442-2445.1997.

Construction and characterization of Escherichia coli genetically engineered for bioremediation of Hg(2+)-contaminated environments

S Chen  1 , D B Wilson
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Construction and characterization of Escherichia coli genetically engineered for bioremediation of Hg(2+)-contaminated environments

S Chen et al. Appl Environ Microbiol. 1997 Jun.

Abstract

Escherichia coli strains were genetically engineered to express an Hg2+ transport system and metallothionein. Overexpression of a glutathione S-transferase fusion protein of Saccharomyces cerevisiae or pea metallothionein significantly increased the bioaccumulation of Hg2+ transported by MerT and MerP and protected the cells from the accumulated Hg2+. The recombinant strains have excellent properties for bioremediation of Hg(2+)-contaminated environments.

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