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. 1997 Apr 11;691(2):277-86.
doi: 10.1016/s0378-4347(96)00458-6.

Determination of agmatine in brain and plasma using high-performance liquid chromatography with fluorescence detection

Y Feng  1 , A E Halaris, J E Piletz
Affiliations

Determination of agmatine in brain and plasma using high-performance liquid chromatography with fluorescence detection

Y Feng et al. J Chromatogr B Biomed Sci Appl. .

Erratum in

  • J Chromatogr B Biomed Sci Appl 1997 Aug 15;696(1):173

Abstract

Decarboxylated arginine, agmatine, is a neurotransmitter candidate for imidazoline receptors. A method is described to measure agmatine in rat brain and human plasma by isocratic high-performance liquid chromatography (HPLC) with fluorescence detection and o-phthalaldehyde derivatization. Quantitation is based on the method of additions of internal agmatine spikes. This assay has sensitivity in the low picomole range and a detection limit of 100 fmol. The correlation coefficient for the agmatine standard curve was 0.999+/-0.001 S.D., and intra- and inter-assay C.V.s were less than 8%. The accuracy of our isocratic method compared favorably with a gradient HPLC protocol, originally developed for bacterial agmatine, which we modified for use with tissues. Agmatine concentrations in rat brain were proportioned similarly to the regional distribution of imidazoline-1 receptors. These methods can be used as reliable research tools in various biological samples.

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