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. 1997 Apr 25;692(1):187-98.
doi: 10.1016/s0378-4347(96)00393-3.

Determination of flunixin in equine urine and serum by capillary electrophoresis

X Gu  1 , M Meleka-Boules, C L Chen, D M Ceska, D M Tiffany
Affiliations

Determination of flunixin in equine urine and serum by capillary electrophoresis

X Gu et al. J Chromatogr B Biomed Sci Appl. .

Abstract

A capillary electrophoresis (CE) and a solid-phase extraction method was developed for the determination of flunixin in equine urine and serum. The suitable CE run conditions were described. The factors affecting flunixin recovery rates were investigated and optimum solid-phase extraction conditions for flunixin in equine urine and serum were established. Limits of detection and quantitation were 3.4 and 5.6 ng/ml for serum and 16.9 and 33.1 ng/ml for urine, respectively. The recoveries exceeded 96% for urine and 79% for serum. Urine samples from race horses and urine and serum samples from a mare administrated with flunixin were analyzed with this procedure.

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