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. 1997 Jun;28(2):298-300.
doi: 10.1002/(sici)1097-0134(199706)28:2<298::aid-prot18>3.3.co;2-3.

Purification and crystallization of Pseudomonas aeruginosa chloramphenicol acetyltransferase

Y Tian  1 , T W Beaman, S L Roderick
Affiliations

Purification and crystallization of Pseudomonas aeruginosa chloramphenicol acetyltransferase

Y Tian et al. Proteins. 1997 Jun.

Abstract

A chloramphenicol acetyltransferase from Pseudomonas aeruginosa genomic DNA has been overexpressed, refolded, purified, and crystallized. Crystals suitable for a three-dimensional x-ray structure determination were obtained from solutions of polyethyleneglycol methyl ether 2000 containing NiCl2 at pH 8.5. These crystals belong to the cubic space group P4(1/3)32 (a = 154.8 A) and diffract x-rays to approximately 3.2 A resolution.

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