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. 1997;83(5):518-21.
doi: 10.1007/s004360050291.

Isolation and partial characterization of a fatty-acid-binding protein from Ascaris suum reproductive tissue

A M Timanova  1 , T Marti, R D Walter, I Y Bankov
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Isolation and partial characterization of a fatty-acid-binding protein from Ascaris suum reproductive tissue

A M Timanova et al. Parasitol Res. 1997.

Abstract

A 12-kDa fatty-acid-binding protein was purified to homogeneity from Ascaris suum reproductive tissue as confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. N-terminal amino-acid-sequence analysis of the protein revealed its identity with the ABA-1 allergen protein isolated from A. suum pseudocoelomic fluid. Fatty-acid binding by the protein from A. suum reproductive tissue was investigated using the Lipidex 1000 assay, which revealed the presence of a single class of fatty-acid-binding sites with an apparent dissociation constant for palmitate of about 0.8 microM.

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