doi: 10.1128/iai.65.7.2970-2974.1997.
A monoclonal antibody directed against the 97-kilodalton gonococcal hemin-binding protein inhibits hemin utilization by Neisseria gonorrhoeae
Affiliations
- PMID: 9199474
- PMCID: PMC175416
- DOI: 10.1128/iai.65.7.2970-2974.1997
Item in Clipboard
A monoclonal antibody directed against the 97-kilodalton gonococcal hemin-binding protein inhibits hemin utilization by Neisseria gonorrhoeae
Infect Immun.
1997 Jul.
Abstract
Neisseria gonorrhoeae expresses two hemin-binding proteins (HmBPs) of 97,000 and 44,000 in molecular weight. A murine monoclonal antibody (MAb) produced against the 97-kDa HmBP from N. gonorrhoeae PID543 specifically inhibited in a concentration-dependent manner the ability of hemin to promote growth. The anti-97-kDa HmBP MAb competitively inhibited binding of the 97-kDa HmBP to a hemin-agarose affinity column. In Western immunoblots, the MAb recognized the 97-kDa homologs from a limited survey of clinical gonococcal isolates. These results support the contention that the 97-kDa HmBP is involved in the gonococcal hemin acquisition pathway.
Similar articles
-
Binding and accumulation of hemin in Neisseria gonorrhoeae.Infect Immun. 1995 Dec;63(12):4634-41. doi: 10.1128/iai.63.12.4634-4641.1995. Infect Immun. 1995. PMID: 7591117 Free PMC article.
-
A 35-kDa co-aggregation factor is a hemin binding protein in Porphyromonas gingivalis.Biochem Biophys Res Commun. 2003 Jan 10;300(2):351-6. doi: 10.1016/s0006-291x(02)02826-7. Biochem Biophys Res Commun. 2003. PMID: 12504090
-
The MF6p/FhHDM-1 major antigen secreted by the trematode parasite Fasciola hepatica is a heme-binding protein.J Biol Chem. 2014 Jan 17;289(3):1441-56. doi: 10.1074/jbc.M113.499517. Epub 2013 Nov 26. J Biol Chem. 2014. PMID: 24280214 Free PMC article.
-
Expression of iron binding proteins and hemin binding activity in the dental pathogen Actinobacillus actinomycetemcomitans.FEMS Microbiol Lett. 1998 Jun 15;163(2):135-42. doi: 10.1111/j.1574-6968.1998.tb13037.x. FEMS Microbiol Lett. 1998. PMID: 9673015
-
Characterization of human-type monoclonal antibodies against reduced form of hemin binding protein 35 from Porphyromonas gingivalis.J Periodontal Res. 2011 Dec;46(6):673-81. doi: 10.1111/j.1600-0765.2011.01389.x. Epub 2011 Jun 6. J Periodontal Res. 2011. PMID: 21644999
Cited by
-
A mutant form of the Neisseria gonorrhoeae pilus secretin protein PilQ allows increased entry of heme and antimicrobial compounds.J Bacteriol. 2004 Feb;186(3):730-9. doi: 10.1128/JB.186.3.730-739.2004. J Bacteriol. 2004. PMID: 14729699 Free PMC article.
-
Molecular characterization of the hemin uptake locus (hmu) from Yersinia pestis and analysis of hmu mutants for hemin and hemoprotein utilization.Infect Immun. 1999 Aug;67(8):3879-92. doi: 10.1128/IAI.67.8.3879-3892.1999. Infect Immun. 1999. PMID: 10417152 Free PMC article.
-
Point mutations in HpuB enable gonococcal HpuA deletion mutants to grow on hemoglobin.J Bacteriol. 2002 Jan;184(2):420-6. doi: 10.1128/JB.184.2.420-426.2002. J Bacteriol. 2002. PMID: 11751818 Free PMC article.
-
Phase variation of hemoglobin utilization in Neisseria gonorrhoeae.Infect Immun. 1998 Mar;66(3):987-93. doi: 10.1128/IAI.66.3.987-993.1998. Infect Immun. 1998. PMID: 9488386 Free PMC article.
-
Neisseria gonorrhoeae heme biosynthetic mutants utilize heme and hemoglobin as a heme source but fail to grow within epithelial cells.Infect Immun. 1998 Nov;66(11):5215-23. doi: 10.1128/IAI.66.11.5215-5223.1998. Infect Immun. 1998. PMID: 9784525 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
