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. 1997;123(5):296-9.
doi: 10.1007/BF01208642.

Changes in N-acetylglucosaminyltransferase III, IV and V in renal cell carcinoma

T Y Zhu  1 , H L Chen, J X Gu, Y F Zhang, Y K Zhang, R A Zhang
Affiliations

Changes in N-acetylglucosaminyltransferase III, IV and V in renal cell carcinoma

T Y Zhu et al. J Cancer Res Clin Oncol. 1997.

Abstract

The activities of N-acetylglucosaminyltransferase (GnT) III, IV and V were determined in 10 cases of renal cell carcinoma (RCC) and compared with the normal kidney cortex (NKC) regions of the same kidney resected from RCC patients. It was found that the GnT III and GnT IV activities decreased consistently in all samples of RCC, while GnT V activity increased, decreased or did not change in different samples. The mean levels of GnT III and GnT IV activities in RCC were found to be very significantly lower than those of NKC on statistical analysis, but the mean value of GnT V activity was almost identical in RCC and NKC. The decrease in GnT activities in RCC were compatible with the decrease in bisecting N-acetylglucosamine (GlcNAc) and antennary number of complex-type N-glycans in gamma-glutamyltranspeptidase (gamma-GT) partially purified from RCCs as studied with concanavalin A (ConA) affinity column chromatography, which showed a decrease of unbound fraction and increase of bound fractions.

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References

    1. Bradford MM (1976) A rapid and sensitive method for quantitation of microquantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248–254 - PubMed
    1. Brewer CF, Bhattacharyya L (1986) Specificity of Concanavalin A binding to asparagine linked glycopeptides. A nuclear magnetic relaxation dispersion study. J Biol Chem 261:7306–7310 - PubMed
    1. Brockhausan I, Narasimhan S, Schachter H (1988) The biosynthesis of highly branched N-glycans, Studies on the sequential pathway and functional role ofN-acetylglucosaminyl transferase I, II, III, IV, V and VI. Biochimie 70:1521–1533 - PubMed
    1. Chen HL, Dong SC, Ju TZ, Yang XP (1995) Effect of retinoic acid on the structure ofN-glycan on the surface of human hepatocarcinoma cells and its enzymatic mechanism. J Cancer Res Clin Oncol 121:397–401 - PMC - PubMed
    1. Dennis JW (1988) Asn-linked oligosaccharide processing and malignant potential. Cancer Survey 7:573–595 - PubMed

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