Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Nov 24;7(6):1553-65.
doi: 10.1093/nar/7.6.1553.

Isolation and partial purification of the major abundant class rat seminal vesicle poly(A+)-messenger RNA

P E Mansson, D B Carter, A B Silverberg, D B Tully, S E Harris
Free PMC article

Isolation and partial purification of the major abundant class rat seminal vesicle poly(A+)-messenger RNA

P E Mansson et al. Nucleic Acids Res. .
Free PMC article

Abstract

Total poly(A(+))-RNA (poly(A(+))-RNA(tot)) was isolated from rat seminal vesicle and its size distribution determined by 70% formamide 5-25% sucrose density analysis. One major peak was resolved in the 10-13 S region and accounted for approximately 35% of the total poly(A(+))-RNA applied. Preparative 1% SDS, 5-20% linear sucrose density gradients also resolved a single major peak in the 11S region (poly(A(+))(11S). Analysis of poly(A(+))-RNA(tot) and poly(A(+))-RNA(11S) under denaturing conditions on 2% agarose gel electrophoresis demonstrated two major components in both poly(A(+))-RNA populations. Size estimations for these components are 620 and 540 NT respectively. (3)H-cDNA was made to both poly(A(+))-RNA(tot) and poly(A(+))-RNA(11S). Back-hybridization of poly(A(+))-RNA(tot) and poly(A(+))-RNA(11S) to their respective (3)H-cDNA revealed a highly abundant class representing 41% and 85% of the sequences in their respective (3)H-cDNA's. The highly abundant class corresponded to 3-5 sequences present in 30,000-50,000 copies/cell. Invitro translation of poly(A(+))-RNA(11S) resulted in two major polypeptides coded for by the 620 NT long and 540 NT long poly(A(+))-RNA respectively.Images

PubMed Disclaimer

References

    1. Biochem J. 1976 Oct 15;160(1):43-8 - PubMed
    1. Cell. 1976 Dec;9(4 PT 2):761-74 - PubMed
    1. Cell. 1979 Aug;17(4):879-87 - PubMed
    1. Biochemistry. 1979 Feb 20;18(4):616-24 - PubMed
    1. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330-4 - PubMed