Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997 Jul 15;25(14):2841-6.
doi: 10.1093/nar/25.14.2841.

Footprint analysis of the bsp RI DNA methyltransferase-DNA interaction

C Finta  1 , A Kiss
Affiliations

Footprint analysis of the bsp RI DNA methyltransferase-DNA interaction

C Finta et al. Nucleic Acids Res. .

Abstract

The interaction between the GGCC-specific Bsp RI DNA methyltransferase (M. Bsp RI) and substrate DNA was studied with footprinting techniques using a DNA fragment that was unmodified on both strands. Footprinting with DNase I revealed an approximately 14 bp protected region. Footprinting with dimethylsulfate detected major groove interactions with the guanine bases of the recognition sequence. Reaction with 1,10-phenanthroline-copper did not show protection, suggesting that minor groove interactions play little role in sequence-specific recognition by M. Bsp RI. Hydroxyl radical footprinting revealed a protected stretch of 6 nt. The hydroxyl radical footprint of M. Bsp RI differs markedly from the the footprint reported for the Hha I and Sss I methyltransferases. The pattern of protection from dimethylsulfate and hydroxyl radicals suggests that the interactions of M. Bsp RI with DNA are similar to those detected in the co-crystal structure of the Hae III methyltransferase.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1978 Sep;5(9):3157-70 - PubMed
    1. J Mol Biol. 1996 Nov 8;263(4):597-606 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Jan;77(1):122-6 - PubMed
    1. Methods Enzymol. 1980;65(1):499-560 - PubMed
    1. J Mol Biol. 1983 Nov 5;170(3):597-610 - PubMed

Publication types

Associated data