Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997 Jul 8;94(14):7595-9.
doi: 10.1073/pnas.94.14.7595.

Induction of host signal transduction pathways by Helicobacter pylori

E D Segal  1 , C Lange, A Covacci, L S Tompkins, S Falkow
Affiliations

Induction of host signal transduction pathways by Helicobacter pylori

E D Segal et al. Proc Natl Acad Sci U S A. .

Abstract

Adherence of Helicobacter pylori to cultured gastric epithelial cells is associated with several cellular events, including the tyrosine phosphorylation of a 145-kDa host protein; the reorganization of the host cell actin and associated cellular proteins, like vasodilator-stimulated phosphoprotein, adjacent to the attached bacterial cell; and the subsequent release of the cytokine, interleukin 8 (IL-8). H. pylori isolated from patients with ulcer disease and gastric cancer contain a DNA insertion, the cag pathogenicity island (PAI), that is not present in bacteria isolated from individuals with asymptomatic infection. Mutations in a number of PAI genes abolish tyrosine phosphorylation and IL-8 synthesis but not the cytoskeletal rearrangements. Kinase inhibition studies suggest there are two distinct pathways operative in stimulating IL-8 release from host cells and one of these H. pylori pathways is independent of the tyrosine phosphorylation step.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Deconvolution IF of spiral H. pylori strains attached to AGS cells (0.2-μm image). Each field is stained for H. pylori (green) and actin (red). Colocalization of H. pylori with actin appears yellow. (A) H. pylori strain G27. (B) H. pylori strain G50. (C) H. pylori mutant 10B4. Imaged on an Applied Precision DeltaVision Deconvolution System.
Figure 2
Figure 2
Immunoblot analysis of AGS cells infected with H. pylori and probed with anti-phosphotyrosine antibody. Lanes: A, extract of AGS cells; B, extract of AGS cells to which H. pylori strain 87A300 had attached for 4 hr; C, extract of AGS cells to which H. pylori strain G104 had attached for 4 hr; D, extract of AGS cells to which H. pylori strain G198 had attached for 4 hr.
Figure 3
Figure 3
Deconvolution IF of spiral H. pylori 87A300 attached to AGS cells (0.2-μm image). Field is stained for H. pylori (blue), actin (red), and VASP (green). Where colocalization of H. pylori and actin occurs, magenta is produced. Where colocalization of actin and VASP occurs, yellow is produced. Imaged on an Applied Precision DeltaVision Deconvolution System.
See this image and copyright information in PMC

References

    1. Logan R P. Lancet. 1994;344:1078–1079. - PubMed
    1. Ghiara P, Marchetti M, Blaser M J, Tummuru M K, Cover T L, Segal E D, Tompkins L S, Rappuoli R. Infect Immun. 1995;63:4154–4160. - PMC - PubMed
    1. McColm, A. A., Bagshaw, J., O’Malley, C. & McLaren, A. (1991) Microb. Ecol. Health Dis. 4, Suppl., S145 (abstr.).
    1. Covacci A, Censini S, Bugnoli M, Petracca R, Burroni D, Macchia G, Massone A, Papini E, Xiang Z, Figura N, Rappuoli R. Proc Natl Acad Sci USA. 1993;90:5791–5795. - PMC - PubMed
    1. Weel J F, van der Hulst R W, Gerrits Y, Roorda P, Feller M, Dankert J, Tytgat G N, van der Ende A. J Infect Dis. 1996;173:1171–1175. - PubMed

Publication types

LinkOut - more resources