Plasma surface modification of artificial corneas for optimal epithelialization

Abstract

We have demonstrated that the optimal surface treatment of a polyvinylalcoholcopolymer hydrogel for epithelial cell migration and proliferation is an argon radio frequency (rf) plasma treatment. The surface chemistry of the material was determined prior to each cellular evaluation, allowing us to compare the biological response with a known surface chemistry. The cellular response was carried out in a consistent manner a minimum of three separate runs. We found that the optimal conditions required culturing the cells under constant rotation. Cells became confluent on argon-plasma-treated surfaces coated under several different reactions pressures, and after 2 weeks they became multilayered. Our experiments demonstrated that cells proliferated and extracellular matrix and adhesion proteins were present only when the surface was treated with an argon rf plasma; acetone- and ammonia-treated surfaces did not yield the desired results. Organ culture experiments further demonstrated the efficacy of the argon-treated surfaces. In these experiments, intact keratoprosthetic devices with modified hydrogel surfaces were implanted into rabbit corneas. The excised corneas containing the devices were cultured, and 3 weeks later, using confocal laser scanning microscopy, confluent epithelium was detected on the modified hydrogel surface. This is the first demonstration that rabbit limbal epithelial cells can migrate onto a synthetic cornea containing a modified hydrogel-treated surface and form a confluent surface of epithelium.