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. 1997 Jul;63(7):2944-8.
doi: 10.1128/aem.63.7.2944-2948.1997.

PCR-mediated detection of acidophilic, bioleaching-associated bacteria

P De Wulf-Durand  1 , L J Bryant, L I Sly
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PCR-mediated detection of acidophilic, bioleaching-associated bacteria

P De Wulf-Durand et al. Appl Environ Microbiol. 1997 Jul.

Abstract

The detection of acidophilic microorganisms from mining environments by culture methods is time consuming and unreliable. Several PCR approaches were developed to amplify small-subunit rRNA sequences from the DNA of six bacterial phylotypes associated with acidic mining environments, permitting the detection of the target DNA at concentrations as low as 10 fg.

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References

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