Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Sep 1;165(3):425-9.
doi: 10.1042/bj1650425.

Partial purification of two lithocholic acid-binding proteins from rat liver 100 000g supernatants

R C Strange, R Cramb, J D Hayes, I W Percy-Robb

Partial purification of two lithocholic acid-binding proteins from rat liver 100 000g supernatants

R C Strange et al. Biochem J. .

Abstract

1. The partial purification of two lithocholic acid-binding proteins from liver 100 000g supernatants is described. 2. Gel-filtration, (NH4)2SO4 fractionation, Ca3(PO4)2 fractionation and ion-exchange chromatography were used. 3. Both proteins exhibited glutathione S-transferase activity; one may be the non-specific anion-binding protein ligandin. 4. Glutathione S-transferase activity of one of the binding proteins was inhibited by lithocholic acid.

PubMed Disclaimer

References

    1. Eur J Biochem. 1976 Aug 16;67(2):583-90 - PubMed
    1. Biochem J. 1977 Mar 15;162(3):659-64 - PubMed
    1. J Physiol. 1966 Oct;186(2):424-38 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 Oct;71(10):3879-82 - PubMed
    1. Eur J Biochem. 1975 Jul 15;55(3):617-23 - PubMed