Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997 Jun;7(3):333-5.
doi: 10.1007/BF02740823.

Efficient preparation of single-stranded DNA for in vitro selection

M J Kujau  1 , S Wölfl
Affiliations

Efficient preparation of single-stranded DNA for in vitro selection

M J Kujau et al. Mol Biotechnol. 1997 Jun.

Abstract

In vitro selection of aptamers requires the reliable enzymatic preparation of large amounts of (+) single-stranded DNA molecules. This can be achieved by selective enzymatic digest of 5'-phosphorylated (-) strands from PCR products, a method already widely used in sequencing of PCR products. Here we present an adaptation of this method to prepare large pools of single-stranded DNA molecules for in vitro selection.

PubMed Disclaimer

References

    1. Nature. 1992 Feb 6;355(6360):564-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Oct;85(20):7652-6 - PubMed
    1. Science. 1990 Aug 3;249(4968):505-10 - PubMed
    1. Biochemistry. 1995 Jul 25;34(29):9599-610 - PubMed
    1. J Bacteriol. 1995 Sep;177(17):4872-80 - PubMed

MeSH terms

Substances

LinkOut - more resources