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. 1997 Jul:115 ( Pt 1):29-32.
doi: 10.1017/s0031182097001091.

A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues

J P Dalton  1 , S R Day, A C Drew, P J Brindley
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A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues

J P Dalton et al. Parasitology. 1997 Jul.

Abstract

A novel method for the isolation of schistosome eggs and miracidia from livers of mice infected with Schistosoma japonicum or S. mansoni is described. The method employed collagenase B to degrade the interstitial matrix of mouse liver tissue, after which the schistosome eggs were separated from the liver cells by 2 single-step density centrifugations through Percoll. Using this procedure sufficient quantities of miracidia were obtained to generate a cDNA library. Southern blot analysis demonstrated that miracidia isolated by this method were free from contaminating host DNA.

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