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. 1979 Feb;87(1):29-49.
doi: 10.3109/13813457909070483.

Membrane-bound mitochondrial DNA: isolation, transcription and protein composition

M Hillar, V Rangayya, B B Jafar, D Chambers, M Vitzu, L E Wyborny

Membrane-bound mitochondrial DNA: isolation, transcription and protein composition

M Hillar et al. Arch Int Physiol Biochim. 1979 Feb.

Abstract

Mitochondrial membrane-bound DNA complex from bovine heart mitochondria lysed in the presence of Triton X-100 was isolated by differential centrifugation. The yield of "nucleoid" is about 30 microgram protein/mg mitochondrial protein. It contains about 3-5 microgram DNA/mg protein and varying amounts of RNA. The heart mitochondrial nucleoid actively synthesizes RNA. The nucleoid fraction contains about sixteen different proteins as evidenced by urea-SDS gel electrophoresis and about twenty-one proteins as evidenced by acid-urea gel electrophoresis. It appears that the nucleoid is attached to the inner membrane since it does contain cytochromes.

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