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. 1997 Jul;4(7):532-8.
doi: 10.1038/nsb0797-532.

Structure of a calpain Ca(2+)-binding domain reveals a novel EF-hand and Ca(2+)-induced conformational changes

H Blanchard  1 , P Grochulski, Y Li, J S Arthur, P L Davies, J S Elce, M Cygler
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Structure of a calpain Ca(2+)-binding domain reveals a novel EF-hand and Ca(2+)-induced conformational changes

H Blanchard et al. Nat Struct Biol. 1997 Jul.

Abstract

The crystal structure of a Ca(2+)-binding domain (dVI) of rat m-calpain has been determined at 2.3 A resolution, both with and without bound Ca2+. The structures reveal a unique fold incorporating five EF-hand motifs per monomer, three of which bind calcium at physiological calcium concentrations, with one showing a novel EF-hand coordination pattern. This investigation gives us a first view of the calcium-induced conformational changes, and consequently an insight into the mechanism of calcium induced activation in calpain. The crystal structures reveal a dVI homodimer which provides a preliminary model for the subunit dimerization in calpain.

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Comment in

  • EF-hands embrace.
    Kretsinger RH. Kretsinger RH. Nat Struct Biol. 1997 Jul;4(7):514-6. doi: 10.1038/nsb0797-514. Nat Struct Biol. 1997. PMID: 9228939 No abstract available.

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