Isoprostanes--prostaglandin-like compounds formed in vivo independently of cyclooxygenase: use as clinical indicators of oxidant damage

Abstract

F2-isoprostanes are prostanoids produced independently of cyclooxygenase by free radical-catalyzed peroxidation of arachidonic acid-containing lipids. Quantification of F2-isoprostanes from biologic fluids and tissues represents an important advance in the detection and measurement of lipid peroxidation in vivo. In addition, efforts to understand both the biophysical effects of isoprostane containing lipids and the biologic effects of free isoprostanes should lead to a better understanding of the mechanisms responsible for oxidant stress-related alterations in homeostasis. Continued application of F2-isoprostane measurement in experimental models of free radical-induced injury and human disease may allow better design and evaluation of antioxidant therapeutic strategies.