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. 1997 May-Jun;91(3):283-5.
doi: 10.1016/s0035-9203(97)90075-4.

Detection and characterization of Leishmania in tissues of patients with post kala-azar dermal leishmaniasis using a specific monoclonal antibody

A Ismail  1 , A Kharazmi, H Permin, A M el Hassan
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Detection and characterization of Leishmania in tissues of patients with post kala-azar dermal leishmaniasis using a specific monoclonal antibody

A Ismail et al. Trans R Soc Trop Med Hyg. 1997 May-Jun.

Abstract

Sections from skin lesions and draining lymph nodes of patients with post kala-azar dermal leishmaniasis were examined using an immunoperoxidase method and a monoclonal antibody directed against Leishmania donovani. Parasites were detected in 22 of 25 biopsies (88%). In parallel sections stained by haematoxylin and eosin, parasites were detected in only 5 of 25 of the biopsies (20%). The demonstration of whole parasites or parasite antigen is diagnostic.

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