Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997 Aug;65(8):3474-7.
doi: 10.1128/iai.65.8.3474-3477.1997.

Adherence of Vibrio cholerae to cultured differentiated human intestinal cells: an in vitro colonization model

J A Bénitez  1 , R G Spelbrink, A Silva, T E Phillips, C M Stanley, M Boesman-Finkelstein, R A Finkelstein
Affiliations

Adherence of Vibrio cholerae to cultured differentiated human intestinal cells: an in vitro colonization model

J A Bénitez et al. Infect Immun. 1997 Aug.

Abstract

Choleragenic vibrios adhered to and multiplied on monolayers of the highly differentiated mucin-secreting cell line HT29-18N2. Their adherence followed first-order kinetics, was dependent on the concentration of vibrios, and was partially inhibited by lipopolysaccharide. Comparison of genetically modified vibrios showed that flagella, an active toxR gene, and the virulence cassette were not essential for initial binding. Inactivation of the hemagglutinin/protease increased binding. This highly differentiated human intestinal cell line provides a versatile new approach for studying major events occurring during intestinal colonization: adherence, multiplication, and detachment.

PubMed Disclaimer

References

    1. Infect Immun. 1976 Aug;14(2):527-47 - PubMed
    1. Microbiology. 1997 Jan;143 ( Pt 1):23-34 - PubMed
    1. J Exp Med. 1988 Oct 1;168(4):1487-92 - PubMed
    1. J Electron Microsc Tech. 1990 Sep;16(1):25-36 - PubMed
    1. J Bacteriol. 1991 Jun;173(11):3311-7 - PubMed

Publication types

LinkOut - more resources