Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 1997 Jul;12(3):235-53.
doi: 10.1006/meth.1997.0476.

Purification of the transcription/repair factor TFIIH and evaluation of its associated activities in vitro

J C Marinoni  1 , M Rossignol, J M Egly
Affiliations
Review

Purification of the transcription/repair factor TFIIH and evaluation of its associated activities in vitro

J C Marinoni et al. Methods. 1997 Jul.

Abstract

We describe here the methodology developed in our laboratory to study the role of TFIIH, a multisubunit protein complex, in the various mechanisms of cell life: transcription, DNA repair, and cell cycle regulation. Protocols are given to purify TFIIH and to study its various enzymatic activities as well as its transcription and nucleotide excision repair activities.

PubMed Disclaimer

Publication types

Substances

LinkOut - more resources