Modulation of cognition-specific cortical activity by gonadal steroids: a positron-emission tomography study in women

Abstract

There is considerable evidence from animal studies that gonadal steroid hormones modulate neuronal activity and affect behavior. To study this in humans directly, we used H215O positron-emission tomography to measure regional cerebral blood flow (rCBF) in young women during three pharmacologically controlled hormonal conditions spanning 4-5 months: ovarian suppression induced by the gonadotropin-releasing hormone agonist leuprolide acetate (Lupron), Lupron plus estradiol replacement, and Lupron plus progesterone replacement. Estradiol and progesterone were administered in a double-blind cross-over design. On each occasion positron-emission tomography scans were performed during (i) the Wisconsin Card Sorting Test, a neuropsychological test that physiologically activates prefrontal cortex (PFC) and an associated cortical network including inferior parietal lobule and posterior inferolateral temporal gyrus, and (ii) a no-delay matching-to-sample sensorimotor control task. During treatment with Lupron alone (i.e., with virtual absence of gonadal steroid hormones), there was marked attenuation of the typical Wisconsin Card Sorting Test activation pattern even though task performance did not change. Most strikingly, there was no rCBF increase in PFC. When either progesterone or estrogen was added to the Lupron regimen, there was normalization of the rCBF activation pattern with augmentation of the parietal and temporal foci and return of the dorsolateral PFC activation. These data directly demonstrate that the hormonal milieu modulates cognition-related neural activity in humans.

Figure 1

Statistical parametric maps showing areas, in red, where cerebral blood flow during the WCS exceeded that during the sensorimotor control task (P < 0.01) for three hormonal conditions: hypogonadism induced by Lupron, Lupron plus progesterone replacement, and Lupron plus estrogen replacement. (Left) Results projected onto three orthogonal two-dimensional planes. (Right) Results for lateral and medial quadrants of the left and right hemispheres projected onto lateral and medial views of a brain.

Figure 2

Number of frontal lobe pixels in which cerebral blood flow during the WCS exceeded that during the sensorimotor control task (P < 0.01) for three hormonal conditions: hypogonadism induced by Lupron, Lupron plus progesterone replacement, and Lupron plus estrogen replacement. PROG, progesterone; ESTR, estrogen.