Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997;2(3):147-55.
doi: 10.1007/BF01682202.

Optimization of the synthesis of peptide combinatorial libraries using a one-pot method

L W Herman  1 , G Tarr, S A Kates
Affiliations

Optimization of the synthesis of peptide combinatorial libraries using a one-pot method

L W Herman et al. Mol Divers. 1997.

Abstract

Conditions for the synthesis of synthetic peptide combinatorial libraries (SPCLs) from mixtures of amino acids were explored. In a one-pot synthesis, the effect of the starting concentrations of amino acids on the resulting library composition was studied, and the optimum balance of amino acids was determined. Protein sequencing, MALDI-TOF, and amino acid analysis were used for the evaluation of the libraries, and their relative merits-are discussed. The effects of continuous-flow automated synthesis instrumentation in conjunction with polyethylene glycol-polystyrene (PEG-PS) graft supports and various cleavage cocktails on the successful synthesis of SPCLs were examined.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):11138-42 - PubMed
    1. J Med Chem. 1994 Aug 19;37(17):2678-85 - PubMed
    1. J Med Chem. 1994 Apr 29;37(9):1233-51 - PubMed
    1. J Med Chem. 1995 Jan 20;38(2):344-52 - PubMed
    1. Int J Pept Protein Res. 1991 Jun;37(6):487-93 - PubMed

MeSH terms