Investigating the cytoskeleton of chicken cardiocytes with the atomic force microscope

Abstract

We have investigated living chicken cardiocytes with an atomic force microscope (AFM). Cytoskeletal structures like stress fibers can easily be imaged with the AFM. Here we have also measured the cell's elastic properties. By taking force curves as a function of lateral position (force mapping) we could compare the elastic properties at different locations of the same cell. In the lamellipodal region investigated here in detail, the elastic moduli range from around 10 up to 200 kPa on top of stress fibers. By degradation with cytochalasin B we can estimate to what extent the elastic properties of this type of cell are determined by the actin network.