The proliferating cell nuclear antigen (PCNA) gene family in Zea mays is composed of two members that have similar expression programmes

Abstract

PCNA is an auxilliary protein for DNA polymerase delta whose function is to increase both polymerase activity and processivity. We have previously reported the isolation of a maize cDNA clone encoding a homologue of PCNA. Here we report the identification of a second maize PCNA cDNA clone. The nucleic acid sequence of both clones is almost identical in the coding sequences, showing 94% identity, but differs by approximately 40% in the 5' and 3' non-translated regions. Maize genomic Southern blots probed with the complete cDNAs and gene-specific probes revealed that maize contains two PCNA genes. Northern blots of RNA extracted from different plant tissues show that both genes are equally expressed in proliferating tissues.