Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997 May;79(5):287-92.
doi: 10.1016/s0300-9084(97)83516-x.

Cloning and overexpression of polypeptide release factor 1 of Thermus thermophilus

K Ito  1 , Y Nakamura
Affiliations

Cloning and overexpression of polypeptide release factor 1 of Thermus thermophilus

K Ito et al. Biochimie. 1997 May.

Abstract

A prfA gene encoding polypeptide release factor RF1 was cloned from Thermus thermophilus. T thermophilus RF1 shares 68% homology with Escherichia coli RF1, and its overproduction reduced readthrough translation of UAG, not of UGA, in the lacZ gene. Rapid purification of T thermophilus RF1 was achieved by T7-RNA polymerase driven overexpression of T thermophilus RF1 protein with a C-terminal histidine tag.

PubMed Disclaimer

Publication types

LinkOut - more resources