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. 1979 Oct 15;57(20):1129-32.
doi: 10.1007/BF01481494.

Solid-phase radioimmunoassay for hepatitis Be antigen (HBeAg)

H E Blum, G Dölken, W Gerok

Solid-phase radioimmunoassay for hepatitis Be antigen (HBeAg)

H E Blum et al. Klin Wochenschr. .

Abstract

A solid-phase radioimmunoassay was developed for the detection of HBeAg and anti-HBe in sera or serum fractions. HBe/sAg positive sera, partially purified HBeAg, partially purified HBsAg, and HBe/sAg negative sera were polymerized in polyacrylamide and compared for their ability to bind 125I-IgG (anti-HBe). Only gels containing HBeAg reacted specifically with the iodinated antibody. The specificity of the binding was confirmed by blocking and inhibition tests using anti-HBe, HBeAg, HBsAg, and negative control sera. The radioimmunoassay allows the specific and quantitative detection of HBeAg and anti-HBe even in the presence of detergents and high salt concentrations.

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