doi: 10.1046/j.1365-313x.1997.12010215.x.
Structure and subcellular localization of a small RNA-binding protein from tobacco
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- PMID: 9263462
- DOI: 10.1046/j.1365-313x.1997.12010215.x
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Structure and subcellular localization of a small RNA-binding protein from tobacco
Plant J.
1997 Jul.
Free article
Abstract
In this study, a cDNA encoding a small RNA-binding protein was isolated from a Nicotiana sylvestris cDNA library. The predicted protein (RGP-3) is 144 amino acid residues long, and contains a consensus sequence-type RNA binding domain (CS-RBD) of 83 amino acids and a short glycine-rich region of 15 amino acids. RGP-3 synthesized in Escherichia coli has high affinity for poly(U). Immunocytochemical analysis indicated that RGP-3 is localized in the nucleoplasm, and that RGP-1b, a related protein reported previously, is localized in the nucleolus. Possible roles of these proteins in pre-mRNA or pre-rRNA processing are discussed.
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