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. 1997 Jun;88(6):600-4.
doi: 10.1111/j.1349-7006.1997.tb00424.x.

Apoptosis induced by NS-398, a selective cyclooxygenase-2 inhibitor, in human colorectal cancer cell lines

A Hara  1 , N Yoshimi, M Niwa, N Ino, H Mori
Affiliations

Apoptosis induced by NS-398, a selective cyclooxygenase-2 inhibitor, in human colorectal cancer cell lines

A Hara et al. Jpn J Cancer Res. 1997 Jun.

Abstract

Recent studies have suggested that apoptosis is a key phenomenon in the chemopreventive action of nonsteroidal antiinflammatory drugs (NSAIDs), which exhibit cancer-preventive and tumor-regressive effects in the human colon. The effect of NS-398, N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide, which is a selective inhibitor of cyclooxygenase-2 (COX-2), on the induction of apoptosis in two human colorectal cancer cell lines (Colo320 and THRC) was determined. The apoptotic ratios (-fold vs. control value) of Colo320 in the presence of 100 microM indomethacin and NS-398 were 3.3 +/- 1.5 and 9.0 +/- 0.94, and those of THRC were 2.3 +/- 0.46 and 7.4 +/- 0.87, respectively. The ability of NS-398 to induce apoptosis is greater than that of indomethacin. Both indomethacin and NS-398 reduced the cell proliferation in a concentration-dependent manner. The IC50 values of NS-398 (54.8 +/- 3.6 and 77.2 +/- 4.9 microM) were significantly lower than those of indomethacin (206.3 +/- 43.0 and 180.3 +/- 22.6 microM) at P < 0.01 in Colo320 and THRC cell lines, respectively. These findings suggest that NS-398, a selective inhibitor of COX-2, is a possible candidate for a chemopreventive agent with a potent apoptosis-inducing effect and low ulcerogenic activity.

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References

    1. ) Thun , M. J. , Namboodiri , M. M. and Heath , C. W , Jr.Aspirin use and reduced risk of fatal colon cancer . N. Engl. J. Med. , 325 , 1593 – 1596 ( 1991. ). - PubMed
    1. ) Giardiello , F. M. , Hamilton , S. R. , Krush , A. J. , Piantadosi , S. , Hylind , L. M. , Celano , P. , Booker , S. V. , Robinson , C. R. and Offerhaus , G. J.Treatment of colonic and rectal adenomas with sulindac in familial adenomatous polyposis . N. Engl. J. Med. , 328 , 1313 – 1316 ( 1993. ). - PubMed
    1. ) Labyle , D. , Fischer , D. , Vielh , P. , Drouhin , P. , Pariente , A. , Bories , C. , Duhamel , O. , Trousset , M. and Attali , P.Sulindac causes regression of rectal polyps in familial adenomatous polyposis . Gastroenterology , 101 , 635 – 639 ( 1991. ). - PubMed
    1. ) Reddy , B. S. , Rao , C. V. , Rivenson , A. and Kelloff , G.Inhibitory effects of aspirin 'on azoxymethane‐induced colon carcinogenesis in F344 rats . Carcinogenesis , 14 , 1493 – 1497 ( 1993. ). - PubMed
    1. ) Rao , C. V. , Rivenson , A. , Simi , B. , Zang , E. , Kelloff , G. , Steele , V. and Reddy , B. S.Chemoprevention of colon carcinogenesis by sulindac, a nonsteroidal anti‐inflammatory agent . Cancer Res. , 55 , 1464 – 1472 ( 1995. ). - PubMed

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